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產(chǎn)品詳情
  • 產(chǎn)品名稱:重組大鼠白細(xì)胞介素-17A蛋白

  • 產(chǎn)品型號:rRtIL-17A
  • 產(chǎn)品廠商:KALANG
  • 產(chǎn)品文檔:
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簡單介紹:
重組大鼠白細(xì)胞介素-17A蛋白與其它公司提供的重組蛋白不同,rRtIL-17A蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白,可表達出對細(xì)胞有毒性、易被蛋白酶降解的蛋白;并獲得具有良好的可溶性,并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效,將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠白細(xì)胞介素-17A蛋白(全長序列)產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹:

重組大鼠白細(xì)胞介素-17A蛋白

Species Rat
Accession Q61453
Unigene Rn.218513.
Source Escherichia coli.
Molecular Weight 重組大鼠白細(xì)胞介素-17A蛋白Approximately 30.0 kDa, a disulfide-linked homodimer of two 133 amino acid polypeptide chains.
Quantity 5μg/25μg/1000μg
AA Sequence AVLIPQSSVC PNAEANNFLQ NVKVNLKVIN SLSSKASSRR PSDYLNRSTS PWTLSRNEDP DRYPSVIWEA QCRHQRCVNA EGKLDHHMNS VLIQQEILVL KREPEKCPFT FRVEKMLVGV GCTCVSSIVR HAS
Purity 重組大鼠白細(xì)胞介素-17A蛋白> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by inducing IL-6 secretion of murine NIH/3T3 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 × 106 IU/mg.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation 重組大鼠白細(xì)胞介素-17A蛋白Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 6.5.
Endotoxin Less than 0.1 EU/μg of rRtIL-17A as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組大鼠白細(xì)胞介素-17A蛋白

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