產(chǎn)品詳情
簡單介紹：
重組鼠KC / CXCL1蛋白與其它公司提供的重組蛋白不同，rMuKC/CXCL1蛋白產(chǎn)品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組鼠KC / CXCL1蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組鼠KC / CXCL1蛋白
| Synonyms | C-X-C motif chemokine 1, GRO-alpha, Platelet-derived growth factor-inducible protein, KC, Secretory protein N51 |
| Species | Murine |
| Accession | P12850 |
| GeneID | 14825 |
| Source | Escherichia coli. |
| Molecular Weight | 重組鼠KC / CXCL1蛋白Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 72 amino acid residues. |
| Quantity | 5μg/20μg/1000μg |
| AA Sequence | APIANELRCQ CLQTMAGIHL KNIQSLKVLP SGPHCTQTEV IATLKNGREA CLDPEAPLVQ KIVQKMLKGV PK |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組鼠KC / CXCL1蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration range of 10-100 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rMuKC/CXCL1 as determined by LAL method. |
| Reconstitution | 重組鼠KC / CXCL1蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組鼠KC / CXCL1蛋白 | |
| SDS-PAGE |
|
| Reference |
1. Haskill S, Peace A, Morris J, et al. 1990. Proc Natl Acad Sci U S A. 87:7732-6. 2. Anisowicz A, Bardwell L, Sager R. 1987. Proc Natl Acad Sci U S A. 84:7188-92. 3. Richmond A, Thomas HG. 1988. J Cell Biochem. 36:185-98. 4. Iida N, Grotendorst GR. 1990. Mol Cell Biol. 10:5596-9. 5. Tsai HH, Frost E, To V, et al. 2002. Cell. 110:373-83. 6. Moser B, Clark-Lewis I, Zwahlen R, et al. 1990. J Exp Med. 171:1797-802. 7. Devalaraja RM, Nanney LB, Du J, et al. 2000. J Invest Dermatol. 115:234-44. |
| Background | Murine CXCL1, also known as KC, is belonging to the CXC chemokine family. It is encoded by the GRO gene now designated CXCL1. The gene for CXCL1 was initially discovered in mouse fibroblasts by plateletderived growth factor. KC is member of the intercrine alpha (chemokine C-X-C) subfamily of chemokines. It is secreted by human melanoma cells, and also expressed by macrophages, neutrophils and epithelial cells. The functional receptor for CXCL1 has been identified as CXCR2. CXCL1 has chemotactic activity for neutrophils, and plays a role in inflammation and wound healing. Amino acid sequence of murine CXCL1 is approximately 60 % identical to the human CXCL1. KC was found to be involved in monocyte arrest on atherosclerotic endothelium and may also play a pathophysiological role in Alzheimer’s disease. |
