產(chǎn)品詳情
簡單介紹：
重組鼠血紅蛋白蛋白與其它公司提供的重組蛋白不同，rMuUteroglobin蛋白產(chǎn)品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組鼠血紅蛋白蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組鼠血紅蛋白蛋白
| Species | Murine |
| Accession | Q06318 |
| GeneID | 22287 |
| Source | Escherichia coli. |
| Molecular Weight | 重組鼠血紅蛋白蛋白Approximately 16.7 kDa, a disulfide-linked homodimeric protein containing two 75 amino acid residues polypeptide. |
| Quantity | 10μg/50μg/1000μg |
| AA Sequence | DICPGFLQVL EALLMESESG YVASLKPFNP GSDLQNAGTQ LKRLVDTLPQ ETRINIMKLT EKILTSPLCK QDLRF |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組鼠血紅蛋白蛋白Fully biologically active when compared to standard. The ED50 as determined by the ability of the immobilized protein to support the adhesion of the A549 human lung carcinoma cells is less than 5.0 μg/ml, corresponding to a specific activity of > 200 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 0.1 EU/μg of rMuUteroglobin as determined by LAL method. |
| Reconstitution | 重組鼠血紅蛋白蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組鼠血紅蛋白蛋白 | |
| SDS-PAGE |
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