產(chǎn)品詳情
簡單介紹：
重組大鼠血管內(nèi)皮生長因子164蛋白與其它公司提供的重組蛋白不同，rRtVEGF164蛋白產(chǎn)品為采用CFS的無細胞麥胚蛋白合成系統(tǒng)表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠血管內(nèi)皮生長因子164蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組大鼠血管內(nèi)皮生長因子164蛋白
| Synonyms | VPF |
| Species | Rat |
| Accession | P16612 |
| GeneID | 83785 |
| Source | 重組大鼠血管內(nèi)皮生長因子164蛋白Escherichia coli. |
| Molecular Weight | Approximately 38.7 kDa, a disulfide-linked homodimeric protein, consisting of two 165 amino acid polypeptide chains with Met at N-terminus. |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR |
| Purity | 重組大鼠血管內(nèi)皮生長因子164蛋白> 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells(HUVEC) is less than 5 ng/ml, corresponding to a specific activity of > 2.0 × 105 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, 300 mM NaCl, pH 8.8. |
| Endotoxin | 重組大鼠血管內(nèi)皮生長因子164蛋白Less than 1 EU/μg of rRtVEGF164 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組大鼠血管內(nèi)皮生長因子164蛋白 | |
| Reference |
1. Leung DW, Cachianes G, Kuang WJ, et al. 1989. Science. 246:1306-9. 2. Byrne AM, Bouchier-Hayes DJ, Harmey JH. 2005. J Cell Mol Med. 9:777-94. 3. Robinson CJ, Stringer SE. 2001. J Cell Sci. 114:853-65. |
| Background | Vascular Endothelial Growth Factor is a sub-family of growth factors produced by cells, which stimulates vasculogenesis and angiogenesis. VEGF's normal function is to create new blood vessels during embryonic development, new blood vessels after injury, muscle following exercise, and new vessels (collateral circulation) to bypass blocked vessels. Mouse and rat express alternately spliced isoforms of 120, 164, and 188 amino acids (a.a.) in length. Recombinant Rat VEGF165 contains 165 amino acids residues (with an met at N- terminal) and it is a disulfide-linked homodimer. In addition, it shares 97 % a.a. sequence identity with corresponding regions of mouse, 88 % with human and bovine, 89 % with porcine and canine, and 90 % with feline and equine VEGF, respectively. |
