產(chǎn)品詳情
簡單介紹：
重組大鼠細(xì)胞因子誘導(dǎo)中性粒細(xì)胞趨化物3 / CXCL2蛋白與其它公司提供的重組蛋白不同，rRtCINC-3/CXCL2蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來的重組蛋白，可表達(dá)出對細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時(shí)獨(dú)有的全自動(dòng)蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠細(xì)胞因子誘導(dǎo)中性粒細(xì)胞趨化物3 / CXCL2蛋白（全長序列）產(chǎn)品可用于Western Blot驗(yàn)證、抗體制備、蛋白檢測、ELISA等試驗(yàn)中.
詳情介紹：
重組大鼠細(xì)胞因子誘導(dǎo)中性粒細(xì)胞趨化物3 / CXCL2蛋白
| Synonyms | MIP-2 |
| Species | Rat |
| Accession | P30348 |
| GeneID | 114105 |
| Source | Escherichia coli. |
| Molecular Weight | 重組大鼠細(xì)胞因子誘導(dǎo)中性粒細(xì)胞趨化物3 / CXCL2蛋白Approximately 7.9 kDa, a single, non-glycosylated polypeptide chain containing 73 amino acids. |
| Quantity | 5μg/25μg/1000μg |
| AA Sequence | VVVASELRCQ CLTTLPRVDF KNIQSLTVTP PGPHCAQTEV IATLKDGHEV CLNPEAPLVQ RIVQKILNKG KAN |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using rat neutrophils is in a concentration range of 10-100 ng/ml. |
| Physical Appearance | 重組大鼠細(xì)胞因子誘導(dǎo)中性粒細(xì)胞趨化物3 / CXCL2蛋白Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rRtCINC-3/CXCL2 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | 重組大鼠細(xì)胞因子誘導(dǎo)中性粒細(xì)胞趨化物3 / CXCL2蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組大鼠細(xì)胞因子誘導(dǎo)中性粒細(xì)胞趨化物3 / CXCL2蛋白 | |
| Reference |
1. Haskill S, Peace A, Morris J, et al. 1990. Proc Natl Acad Sci U S A. 87:7732-6. 2. Tsai HH, Frost E, To V, et al. 2002. Cell. 110:373-83. 3. Wolpe SD, Sherry B, Juers D, et al. 1989. Proc Natl Acad Sci U S A. 86:612-6. 4. Iida N, Grotendorst GR. 1990. Mol Cell Biol. 10:5596-9. 5. Pelus LM, Fukuda S. 2006. Exp Hematol. 34:1010-20. |
| Background | Rat CXCL2 also named CINC-3, belongs to the CXC chemokine family. It is encoded by the gene CXCL2 and shares 90 % amino acid sequence with CXCL1/GROα. It is member of the intercrine alpha (chemokine C-X-C) subfamily of chemokines. This chemokine is expressed by cytokine-stimulated rat alveolar macrophages and fibroblasts. The functional receptor for CXCL2 has been identified as CXCR2. CXCL2 is chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells. Similar to other GRO proteins, CXCL2 is potent neutrophil attractants and activators. In addition, it is also active toward basophils. The amino acid sequence of rat CXCL2 is 88 % identical to murine CXCL2. |
