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產(chǎn)品名稱：重組人類印度刺猬Cys28IleIle蛋白
產(chǎn)品型號：rHuIHH C28II
產(chǎn)品廠商：KALANG
產(chǎn)品文檔：

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簡單介紹：

重組人類印度刺猬Cys28IleIle蛋白與其它公司提供的重組蛋白不同，rHuIHH C28II蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來的重組蛋白，可表達(dá)出對細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人類印度刺猬Cys28IleIle蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.

詳情介紹：

重組人類印度刺猬Cys28IleIle蛋白

Synonyms HHG-2

Species Human

Accession Q14623

GeneID 3549

Source Escherichia coli.

Molecular Weight 重組人類印度刺猬Cys28IleIle蛋白Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 176 amino acids.

Quantity 5μg/25μg/1000μg

AA Sequence IIGPGRVVGS RRRPPRKLVP LAYKQFSPNV PEKTLGASGR YEGKIARSSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDRLNSLAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRNKYGLL ARLAVEAGFD WVYYESKAHV HCSVKSEHSA AAKTGG

Purity > 96 % by SDS-PAGE and HPLC analyses.

Biological Activity 重組人類印度刺猬Cys28IleIle蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by its ability to induce alkaline phosphatase production by C3H10T1/2(CCL-226) cells is 3.0-10 μg/ml.

Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4.

Endotoxin Less than 1 EU/μg of rHuIHH C28II as determined by LAL method.

Reconstitution 重組人類印度刺猬Cys28IleIle蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.

重組人類印度刺猬Cys28IleIle蛋白

Reference 1. Kumar S, Balczarek KA, Lai ZC. 1996. Genetics. 142:965-72.
2. Marigo V, Roberts DJ, Lee SM, et al. 1995. Genomics. 28:44-51.
3. Vortkamp A, Lee K, Lanske B, et al. 1996. Science. 273:613-22.

Background IHH, encoded by the IHH gene in humans, belongs to the mammalian hedgehog family. It is expressed in ***** kidney and liver. The function of IHH is involved in chondrocyte differentiation, proliferation and maturation especially during endochondral ossification. It regulates its effects by feedback control of parathyroid hormone-related peptide (PTHrP). IHH is also involved in yolk sac vasculogenesis, playing an important role in differentiation of epiblast cells into endothelial and red blood cells. Human IHH shares 100 % amino acid sequence identity with murine.

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Synonyms	HHG-2
Species	Human
Accession	Q14623
GeneID	3549
Source	Escherichia coli.
Molecular Weight	重組人類印度刺猬Cys28IleIle蛋白Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 176 amino acids.
Quantity	5μg/25μg/1000μg
AA Sequence	IIGPGRVVGS RRRPPRKLVP LAYKQFSPNV PEKTLGASGR YEGKIARSSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDRLNSLAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRNKYGLL ARLAVEAGFD WVYYESKAHV HCSVKSEHSA AAKTGG
Purity	> 96 % by SDS-PAGE and HPLC analyses.
Biological Activity	重組人類印度刺猬Cys28IleIle蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by its ability to induce alkaline phosphatase production by C3H10T1/2(CCL-226) cells is 3.0-10 μg/ml.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 1 × PBS, pH 7.4.
Endotoxin	Less than 1 EU/μg of rHuIHH C28II as determined by LAL method.
Reconstitution	重組人類印度刺猬Cys28IleIle蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重組人類印度刺猬Cys28IleIle蛋白
Reference	1. Kumar S, Balczarek KA, Lai ZC. 1996. Genetics. 142:965-72. 2. Marigo V, Roberts DJ, Lee SM, et al. 1995. Genomics. 28:44-51. 3. Vortkamp A, Lee K, Lanske B, et al. 1996. Science. 273:613-22.
Background	IHH, encoded by the IHH gene in humans, belongs to the mammalian hedgehog family. It is expressed in ***** kidney and liver. The function of IHH is involved in chondrocyte differentiation, proliferation and maturation especially during endochondral ossification. It regulates its effects by feedback control of parathyroid hormone-related peptide (PTHrP). IHH is also involved in yolk sac vasculogenesis, playing an important role in differentiation of epiblast cells into endothelial and red blood cells. Human IHH shares 100 % amino acid sequence identity with murine.

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產(chǎn)品名稱：重組人類印度刺猬Cys28IleIle蛋白